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Journal of Clinical Microbiology, July 2008, p. 2402-2405, Vol. 46, No. 7
0095-1137/08/$08.00+0     doi:10.1128/JCM.00655-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Molecular Characterization of Porcine Picobirnaviruses and Development of a Specific Reverse Transcription-PCR Assay{triangledown} ,{dagger}

Gabriela M. Carruyo,1,2 Guaniri Mateu,1 Laura C. Martínez,3 Flor H. Pujol,1 Silvia V. Nates,3 Ferdinando Liprandi,1 and Juan E. Ludert1*

Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas, Caracas,1 Facultad de Ciencias Veterinarias, La Universidad del Zulia, Maracaibo, Venezuela,2 Facultad de Ciencias Médicas, Universidad Nacional de Córdoba, Córdoba, Argentina3

Received 7 April 2008/ Returned for modification 8 May 2008/ Accepted 20 May 2008

The molecular characterization of partial- length genomic segment 2 of porcine picobirnavirus (PBV) strains and the development of a specific reverse transcription-PCR (RT-PCR) assay for detection of virus in feces are reported. Phylogenetic analysis indicated that the studied porcine isolates were more closely related (>85% identity) to human PBV belonging to genogroup I than to the other porcine PBV described so far. Analysis by RT-PCR and polyacrylamide gel electrophoresis of fecal samples collected in Venezuela and Argentina showed that PBV circulate at high frequencies in piglets.

* Corresponding author. Present address: Departamento de Infectómica y Patogénesis Molecular, Centro de Investigación y Estudios Avanzados (CINVESATV) del I.P.N., Av. I.P.N. 2508, Col. San Pedro Zacatenco, Mexico City 07360, Mexico. Phone: 52-55-57473800. Fax: 52-55-57473377. E-mail: jeludert{at}gmail.com

{triangledown} Published ahead of print on 28 May 2008.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

Journal of Clinical Microbiology, July 2008, p. 2402-2405, Vol. 46, No. 7
0095-1137/08/$08.00+0     doi:10.1128/JCM.00655-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ghosh, S., Kobayashi, N., Nagashima, S., Naik, T. N. (2009). Molecular characterization of full-length genomic segment 2 of a bovine picobirnavirus (PBV) strain: evidence for high genetic diversity with genogroup I PBVs. J. Gen. Virol. 90: 2519-2524 [Abstract] [Full Text]  
  • Symonds, E. M., Griffin, D. W., Breitbart, M. (2009). Eukaryotic Viruses in Wastewater Samples from the United States. Appl. Environ. Microbiol. 75: 1402-1409 [Abstract] [Full Text]  


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