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Journal of Clinical Microbiology, August 2008, p. 2519-2524, Vol. 46, No. 8
0095-1137/08/$08.00+0     doi:10.1128/JCM.00277-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Detection of All Known Parechoviruses by Real-Time PCR{triangledown}

W. Allan Nix,1 Kaija Maher,1 E. Susanne Johansson,2 Bo Niklasson,3 A. Michael Lindberg,2 Mark A. Pallansch,1 and M. Steven Oberste1*

Polio and Picornavirus Laboratory Branch, Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333,1 University of Kalmar, Kalmar, Sweden,2 Apodemus AB, Stockholm, Sweden3

Received 11 February 2008/ Returned for modification 28 March 2008/ Accepted 22 May 2008

The Parechovirus genus of the Picornaviridae family contains two species, Human parechovirus (HPeV) and Ljungan virus (LV). The HPeVs (including the former echoviruses 22 and 23, now HPeV type 1 (HPeV1) and HPeV2, respectively) cause a wide spectrum of disease, including aseptic meningitis, gastroenteritis, encephalitis, acute respiratory illness, and neonatal sepsis-like disease. The LVs were isolated from bank voles in Sweden during a search for an infectious agent linked to fatal myocarditis cases in humans. Because of the decline in use of cell culture and neutralization to investigate enterovirus-like disease, very few laboratories currently have the capability to test for parechoviruses. We have developed a real-time reverse transcription-PCR (RT-PCR) assay for detection of all known members of the genus Parechovirus. The assay targets the conserved regions in the 5' nontranslated region (5'NTR) of the parechovirus genome and can detect both HPeVs and LVs, unlike other published parechovirus 5' NTR assays, which only detect known HPeVs or only LVs. HPeV and LV can be differentiated by sequencing the 5'NTR real-time RT-PCR amplicon, when needed. The assay is approximately 100 times more sensitive than cell culture and may be used to test original clinical specimens. The availability of a broad-specificity PCR method should facilitate the detection of new human parechoviruses, as well as new parechoviruses in other mammalian species, and provide an opportunity to investigate the role of these viruses in human and animal disease.

* Corresponding author. Mailing address: Centers for Disease Control and Prevention, 1600 Clifton Road, N.E., Mailstop G-17, Atlanta, GA 30333. Phone: (404) 639-5497. Fax: (404) 639-4011. E-mail: soberste{at}cdc.gov

{triangledown} Published ahead of print on 4 June 2008.

Journal of Clinical Microbiology, August 2008, p. 2519-2524, Vol. 46, No. 8
0095-1137/08/$08.00+0     doi:10.1128/JCM.00277-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Harvala, H., Robertson, I., McWilliam Leitch, E. C., Benschop, K., Wolthers, K. C., Templeton, K., Simmonds, P. (2008). Epidemiology and Clinical Associations of Human Parechovirus Respiratory Infections. J. Clin. Microbiol. 46: 3446-3453 [Abstract] [Full Text]  


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