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Journal of Clinical Microbiology, August 2008, p. 2700-2706, Vol. 46, No. 8
0095-1137/08/$08.00+0     doi:10.1128/JCM.00370-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Multicenter Comparison of PCR Assays for Detection of Human Herpesvirus 6 DNA in Serum{triangledown}

Louis Flamand,1,2* Annie Gravel,1 David Boutolleau,3 Roberto Alvarez-Lafuente,4 Steve Jacobson,5 Mauro S. Malnati,6 Debra Kohn,7 Yi-Wei Tang,8 Tetsushi Yoshikawa,9 and Dharam Ablashi10

Rheumatology and Immunology Research Center, CHUL Research Center,1 Department of Anatomy and Physiology, Faculty of Medicine, Laval University, Quebec, Quebec, Canada,2 Department of Virology, University Pierre et Marie Curie-Paris 6, EA2387, Groupe Hospitalier Pitié-Salpêtrière, Paris, France,3 Servicio de Neurología, Hospital Clínico San Carlos, c/Profesor Martín Lagos s/n, 28040 Madrid, Spain,4 Viral Immunology Section, NINDS/NIH, 9000 Rockville Pike, Building 10, Room 5B-16, Bethesda, Maryland 20892,5 Unit of Human Virology, DIBIT, San Raffele Scientific Institute, 20132 Milan, Italy,6 Department of Clinical Pathology, Cleveland Clinic, Cleveland, Ohio 44195,7 Molecular Infectious Diseases Laboratory, 4605 TVC, Vanderbilt University Medical Center, Nashville, Tennessee 37232-5310,8 Department of Medical Information Technology, Fujita Health University College, Toyoake, Aichi, Japan,9 HHV-6 Foundation, 277 San Ysidro Road, Santa Barbara, California 93108,10

Received 23 February 2008/ Returned for modification 10 April 2008/ Accepted 30 May 2008

Human herpesvirus 6 (HHV-6) is a ubiquitous virus with which infections have been associated with pathologies ranging from delayed bone marrow engraftment to a variety of neurological diseases. The lack of a standardized assay that can be used to detect and estimate HHV-6 DNA contents in various clinical specimens can lead and has led to discordant results among investigators and on the potential association of HHV-6 to diseases. To identify the most reliable and sensitive assays, an identical set of 11 coded serum samples spiked with various quantities of the HHV-6A variant (range, 4 to 400,000 genome copies/ml) was sent to eight independent laboratories around the world. Each laboratory was asked to estimate the HHV-6 DNA content by use of its own protocols and assays. Among the various assays, three TaqMan-based real-time PCR assays yielded quantities that were closest to the quantity of HHV-6 that had been spiked. To provide better homogeneity between the results from the different laboratories working on HHV-6, we propose that investigators interested in quantifying HHV-6 in clinical samples adopt one of these assays.

* Corresponding author. Mailing address: Rheumatology and Immunology Research Center, CHUL Research Center, Room T1-49, 2705 Laurier Blvd., Quebec, Quebec, Canada G1V 4G2. Phone: (418) 656-4141, ext. 46164. Fax: (418) 654-2765. E-mail: louis.flamand{at}crchul.ulaval.ca

{triangledown} Published ahead of print on 11 June 2008.

Journal of Clinical Microbiology, August 2008, p. 2700-2706, Vol. 46, No. 8
0095-1137/08/$08.00+0     doi:10.1128/JCM.00370-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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