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Journal of Clinical Microbiology, August 2008, p. 2786-2789, Vol. 46, No. 8
0095-1137/08/$08.00+0     doi:10.1128/JCM.02177-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Herpes Simplex Virus Type 2 Displays Atypical Melting-Temperature Profiles at Low Viral Titers{triangledown}

Jason J. LeBlanc,1 Sarah Campbell,1 Janice Pettipas,1 Todd F. Hatchette,1,2 and Ross J. Davidson1,2*

Department of Pathology and Laboratory Medicine, Queen Elizabeth II Health Sciences Center, Halifax, Nova Scotia, Canada,1 Department of Pathology, Dalhousie University, Halifax, Nova Scotia, Canada2

Received 9 November 2007/ Returned for modification 21 May 2008/ Accepted 27 May 2008

Real-time PCR is a powerful tool for the detection and typing of herpes simplex virus (HSV). HSV types 1 and 2 can be distinguished by using the differences in the melting temperatures (Tms) of the hybridization probes. The efficacy of Tm profiling with low DNA concentrations was evaluated in this study.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Room 309, MacKenzie Building, Queen Elizabeth II Health Sciences Centre, 5788 University Avenue, Halifax, Nova Scotia B3H 1V8, Canada. Phone: (902) 473-5520. Fax: (902) 473-4432. E-mail: Ross.Davidson{at}cdha.nshealth.ca

{triangledown} Published ahead of print on 4 June 2008.

Journal of Clinical Microbiology, August 2008, p. 2786-2789, Vol. 46, No. 8
0095-1137/08/$08.00+0     doi:10.1128/JCM.02177-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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