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Journal of Clinical Microbiology, August 2008, p. 2800-2804, Vol. 46, No. 8
0095-1137/08/$08.00+0     doi:10.1128/JCM.00152-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Common Strains of Escherichia coli{triangledown}

Joshua Hill,1 Shilpa Beriwal,1 Ishwad Chandra,1 Vinod K. Paul,2 Aarti Kapil,3 Tripti Singh,1 Robert M. Wadowsky,4 Vinita Singh,5 Ankur Goyal,1 Timo Jahnukainen,1,{dagger} James R. Johnson,6 Phillip I. Tarr,7 and Abhay Vats1*

Department of Pediatrics, Children's Hospital of Pittsburgh, 3705 Fifth Avenue, Pittsburgh, Pennsylvania 15213,1 Department of Pediatrics, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India,2 Department of Microbiology, All India Institute of Medical Sciences, Ansari Nagar, New Delhi 110029, India,3 Department of Pathology, Children's Hospital of Pittsburgh, 3705 Fifth Avenue, Pittsburgh, Pennsylvania 15213,4 Department of Molecular Diagnostics, Atharva BioSciences, New Delhi 110029, India,5 Department of Medicine, VA Medical Center, Veterans Affairs Medical Center, University of Minnesota, 1 Veterans Drive, Minneapolis, Minnesota 55417,6 Department of Pediatrics and Department of Molecular Microbiology, Washington University School of Medicine, 660 South Euclid Avenue, St. Louis, Missouri 631107

Received 25 January 2008/ Returned for modification 6 March 2008/ Accepted 31 May 2008

We developed a highly sensitive and specific LAMP assay for Escherichia coli. It does not require DNA extraction and can detect as few as 10 copies. It detected all 36 of 36 E. coli isolates and all 22 urine samples (out of 89 samples tested) that had E. coli. This assay is rapid, low in cost, and simple to perform.

* Corresponding author. Mailing address: Department of Pediatrics, Children's Hospital of Pittsburgh, 3705 Fifth Avenue, Pittsburgh, PA 15213. Phone: (412) 692-5182. Fax: (412) 692-7443. E-mail: abhay.vats{at}chp.edu

{triangledown} Published ahead of print on 11 June 2008.

{dagger} Present address: Hospital for Children and Adolescents, University of Helsinki, PB 281, 00029 Helsinki, Finland.

Journal of Clinical Microbiology, August 2008, p. 2800-2804, Vol. 46, No. 8
0095-1137/08/$08.00+0     doi:10.1128/JCM.00152-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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