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Journal of Clinical Microbiology, August 2008, p. 2805-2808, Vol. 46, No. 8
0095-1137/08/$08.00+0     doi:10.1128/JCM.00071-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Characterization of Clonal Relatedness among the Natural Population of Staphylococcus aureus Strains by Using spa Sequence Typing and the BURP (Based upon Repeat Patterns) Algorithm{triangledown}

Alexander Mellmann,1* Thomas Weniger,2 Christoph Berssenbrügge,1 Ursula Keckevoet,1 Alexander W. Friedrich,1 Dag Harmsen,2 and Hajo Grundmann3

Institute for Hygiene,1 Department of Periodontology, University Hospital Münster, D-48149 Münster, Germany,2 Centre for Infectious Diseases Epidemiology, National Institute for Public Health and the Environment, Bilthoven 3720BA, The Netherlands3

Received 14 January 2008/ Returned for modification 27 March 2008/ Accepted 27 May 2008

We evaluated the BURP (based upon repeat patterns) algorithm, which relies on sequencing of the Staphylococcus aureus protein A gene (spa), for its ability to infer clonal relatedness within a population of 110 wild-type strains. BURP clustering of the resulting 66 spa types was highly concordant with multilocus sequence typing (96.5% concordance) and pulsed-field gel electrophoresis (94.9%).

* Corresponding author. Mailing address: Institute for Hygiene, University Hospital Münster, Robert-Koch-Str. 41, 48149 Münster, Germany. Phone: 49 (0) 251-83 52316. Fax: 49 (0) 251-83 55688. E-mail: mellmann{at}uni-muenster.de

{triangledown} Published ahead of print on 4 June 2008.

Journal of Clinical Microbiology, August 2008, p. 2805-2808, Vol. 46, No. 8
0095-1137/08/$08.00+0     doi:10.1128/JCM.00071-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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