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Journal of Clinical Microbiology, September 2008, p. 3094-3096, Vol. 46, No. 9
0095-1137/08/$08.00+0     doi:10.1128/JCM.00945-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Impact of Diversity of Colonizing Strains on Strategies for Sampling Escherichia coli from Fecal Specimens {triangledown}

Ebbing Lautenbach,1,2,3,4* Warren B. Bilker,2,3,4 Pam Tolomeo,3 and Joel N. Maslow1,4,5

Division of Infectious Diseases of the Department of Medicine,1 Department of Biostatistics and Epidemiology,2 Center for Clinical Epidemiology and Biostatistics,3 Center for Education and Research on Therapeutics, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania,4 Section of Infectious Diseases, Department of Veterans Affairs, Philadelphia, Pennsylvania5

Received 17 May 2008/ Returned for modification 3 July 2008/ Accepted 11 July 2008

Of 49 subjects, 21 were colonized with more than one strain of Escherichia coli and 12 subjects had at least one strain present in fewer than 20% of colonies. The ability to accurately characterize E. coli strain diversity is directly related to the number of colonies sampled and the underlying prevalence of the strain.


* Corresponding author. Mailing address: University of Pennsylvania School of Medicine, Center for Clinical Epidemiology and Biostatistics, 825 Blockley Hall, 423 Guardian Drive, Philadelphia, PA 19104-6021. Phone: (215) 898-6977. Fax: (215) 573-5315. E-mail: ebbing{at}mail.med.upenn.edu

{triangledown} Published ahead of print on 23 July 2008.


Journal of Clinical Microbiology, September 2008, p. 3094-3096, Vol. 46, No. 9
0095-1137/08/$08.00+0     doi:10.1128/JCM.00945-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.