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Journal of Clinical Microbiology, September 2008, p. 3116-3118, Vol. 46, No. 9
0095-1137/08/$08.00+0     doi:10.1128/JCM.00440-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Evaluation of Three Real-Time PCR Assays for Detection of Mycoplasma pneumoniae in an Outbreak Investigation{triangledown}

Jonas M. Winchell,* Kathleen A. Thurman, Stephanie L. Mitchell, W. Lanier Thacker, and Barry S. Fields

Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia

Received 5 March 2008/ Returned for modification 4 May 2008/ Accepted 24 June 2008

We compared the performances of three recently optimized real-time PCR assays derived from distinct genomic regions of Mycoplasma pneumoniae during an outbreak. Comprehensive evaluation established that a newly described toxin gene represents a superior target for detecting M. pneumoniae DNA in clinical specimens, although use of multiple targets may increase testing confidence.

* Corresponding author. Mailing address: Centers for Disease Control and Prevention, 1600 Clifton Rd. N.E., MS G-03, Atlanta, GA 30333. Phone: (404) 639-4921. Fax: (404) 639-4215. E-mail: jwinchell{at}cdc.gov

{triangledown} Published ahead of print on 9 July 2008.

Journal of Clinical Microbiology, September 2008, p. 3116-3118, Vol. 46, No. 9
0095-1137/08/$08.00+0     doi:10.1128/JCM.00440-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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  • Dumke, R., Jacobs, E. (2009). Comparison of Commercial and In-House Real-Time PCR Assays Used for Detection of Mycoplasma pneumoniae. J. Clin. Microbiol. 47: 441-444 [Abstract] [Full Text]  
  • Wolff, B. J., Thacker, W. L., Schwartz, S. B., Winchell, J. M. (2008). Detection of Macrolide Resistance in Mycoplasma pneumoniae by Real-Time PCR and High-Resolution Melt Analysis. Antimicrob. Agents Chemother. 52: 3542-3549 [Abstract] [Full Text]  


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