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Journal of Clinical Microbiology, September 2008, p. 3139-3143, Vol. 46, No. 9
0095-1137/08/$08.00+0     doi:10.1128/JCM.00275-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Molecular Method for Discrimination between Francisella tularensis and Francisella-Like Endosymbionts{triangledown}

Raquel Escudero,1 A. Toledo,1,2 Horacio Gil,1 Katarina Kovácsová,1 Manuela Rodríguez-Vargas,1 Isabel Jado,1 Cristina García-Amil,1 Bruno Lobo,1 Mangesh Bhide,1,{dagger} and Pedro Anda1*

Laboratorio de Espiroquetas y Patógenos Especiales, Servicio de Bacteriología, Centro Nacional de Microbiología, Majadahonda, Madrid, Spain,1 Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain2

Received 11 February 2008/ Returned for modification 31 March 2008/ Accepted 2 July 2008

Environmental studies on the distribution of Francisella spp. are hampered by the frequency of Francisella-like endosymbionts that can produce a misleading positive result. A new, efficient molecular method for detection of Francisella tularensis and its discrimination from Francisella-like endosymbionts, as well as two variants associated with human disease (unusual F. tularensis strain FnSp1 and F. tularensis subsp. novicida-like strain 3523), is described. The method is highly specific and sensitive, detecting up to one plasmid copy or 10 genome equivalents.

* Corresponding author. Mailing address: Laboratorio de Espiroquetas y Patógenos Especiales, Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220-Majadahonda, Madrid, Spain. Phone: 34 91 822 3953. Fax: 34 91 509 7966. E-mail: panda{at}isciii.es

{triangledown} Published ahead of print on 23 July 2008.

{dagger} Present address: Laboratory of Biomedical Microbiology and Immunology, University of Veterinary Medicine, 04181, Kosice, Slovakia.

Journal of Clinical Microbiology, September 2008, p. 3139-3143, Vol. 46, No. 9
0095-1137/08/$08.00+0     doi:10.1128/JCM.00275-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Toledo, A., Olmeda, A. S., Escudero, R., Jado, I., Valcarcel, F., Casado-Nistal, M. A., Rodriguez-Vargas, M., Gil, H., Anda, P. (2009). Tick-Borne Zoonotic Bacteria in Ticks Collected from Central Spain. Am J Trop Med Hyg 81: 67-74 [Abstract] [Full Text]  


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