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Journal of Clinical Microbiology, September 2008, p. 3147-3151, Vol. 46, No. 9
0095-1137/08/$08.00+0     doi:10.1128/JCM.00861-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Novel Multiple-Locus Variable-Number Tandem-Repeat Analysis Method for Rapid Molecular Typing of Human Staphylococcus aureus{triangledown}

Risma Ikawaty, Rob J. L. Willems, Adrienne T. A. Box, Jan Verhoef, and Ad C. Fluit*

Department of Medical Microbiology, University Medical Centre Utrecht, Utrecht, The Netherlands

Received 5 May 2008/ Returned for modification 18 June 2008/ Accepted 14 July 2008

We evaluated the use of a novel multiple-locus variable-number tandem-repeat analysis (MLVA) method for typing of human Staphylococcus aureus. For a total of 150 clinical isolates, MLVA demonstrated the highest discriminatory power. MLVA correctly assigned isolates to outbreaks or identified isolates as unlinked. MLVA is a rapid and simple method for the epidemiological typing of S. aureus.

* Corresponding author. Mailing address: Department of Medical Microbiology, University Medical Centre Utrecht, Room G04.614, P.O. Box 85500, Utrecht 3584 CX, The Netherlands. Phone: 31887557630. Fax: 31302541770. E-mail: A.C.Fluit{at}umcutrecht.nl

{triangledown} Published ahead of print on 23 July 2008.

Journal of Clinical Microbiology, September 2008, p. 3147-3151, Vol. 46, No. 9
0095-1137/08/$08.00+0     doi:10.1128/JCM.00861-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pourcel, C., Hormigos, K., Onteniente, L., Sakwinska, O., Deurenberg, R. H., Vergnaud, G. (2009). Improved Multiple-Locus Variable-Number Tandem-Repeat Assay for Staphylococcus aureus Genotyping, Providing a Highly Informative Technique Together with Strong Phylogenetic Value. J. Clin. Microbiol. 47: 3121-3128 [Abstract] [Full Text]  
  • Conceicao, T., Aires de Sousa, M., de Lencastre, H. (2009). Staphylococcal Interspersed Repeat Unit Typing of Staphylococcus aureus: Evaluation of a New Multilocus Variable-Number Tandem-Repeat Analysis Typing Method. J. Clin. Microbiol. 47: 1300-1308 [Abstract] [Full Text]  


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