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Journal of Clinical Microbiology, January 2009, p. 134-141, Vol. 47, No. 1
0095-1137/09/$08.00+0     doi:10.1128/JCM.01183-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Borrelia carolinensis sp. nov., a New (14th) Member of the Borrelia burgdorferi Sensu Lato Complex from the Southeastern Region of the United States {triangledown}

Nataliia Rudenko,1,2,3* Maryna Golovchenko,1,2,3 Libor Grubhoffer,1,2 and James H. Oliver Jr.3

Biology Centre, Institute of Parasitology AS CR,1 Faculty of Sciences, University of South Bohemia, Ceské Budejovice 37005, Czech Republic,2 Georgia Southern University, Institute of Arthropodology and Parasitology, Statesboro, Georgia 30460-80563

Received 23 June 2008/ Returned for modification 30 September 2008/ Accepted 11 November 2008

Approximately 118 Borrelia isolates were cultured from a variety of rodents, birds, and ticks collected in the southern United States. In addition to a highly diverse group of Borrelia bissettii strains and a homogenous group of Borrelia burgdorferi sensu stricto strains, a group of 16 isolates with unusual characteristics was found. The isolates were cultured from ear biopsy samples of the rodents Peromyscus gossypinus and Neotoma floridana trapped at five localities in South Carolina. A multilocus sequence analysis of the rrf-rrl intergenic spacer, 16S rRNA, fla, ospA, and p66 genes were used to clarify the taxonomic status of the new group of B. burgdorferi sensu lato isolates. Thirteen species of the B. burgdorferi sensu lato complex were used as controls. Unique restriction fragment length polymorphism patterns of the rrf-rrl intergenic spacer region and fla gene were recognized. Unique signature nucleotides were also found in the 16S rRNA gene. A phylogenetic analysis shows that the 16 new isolates cluster together but separately from the other species in the B. burgdorferi sensu lato complex. Our data strongly support the recognition of the 16 isolates as a new B. burgdorferi sensu lato species. We propose to name this genospecies "Borrelia carolinensis" with respect to the place of its currently known geographic location.

* Corresponding author. Mailing address: Biology Centre, Institute of Parasitology, Academy of Sciences of the Czech Republic, Branisovská 31, CZ-37005, Ceské Budejovice, Czech Republic. Phone: 421 38 777 5446. Fax: 421 38 531 0388. E-mail: natasha{at}paru.cas.cz

{triangledown} Published ahead of print on 19 November 2008.

Journal of Clinical Microbiology, January 2009, p. 134-141, Vol. 47, No. 1
0095-1137/09/$08.00+0     doi:10.1128/JCM.01183-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



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