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Journal of Clinical Microbiology, January 2009, p. 247-248, Vol. 47, No. 1
0095-1137/09/$08.00+0     doi:10.1128/JCM.01241-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Identification of Candida albicans and Candida glabrata within 1.5 Hours Directly from Positive Blood Culture Bottles with a Shortened Peptide Nucleic Acid Fluorescence In Situ Hybridization Protocol{triangledown}

Michael Gherna and William G. Merz*

Department of Pathology, the Johns Hopkins Medical Institutions, Baltimore, Maryland

Received 1 July 2008/ Returned for modification 8 August 2008/ Accepted 28 October 2008

Candida albicans and Candida glabrata can be identified in blood culture bottles within 2.5 h using peptide nucleic acid fluorescence in situ hybridization. A 1.25-h protocol was compared to the standard with 40 positive (clinical and spiked) blood culture bottles tested in batches of 5. All C. albicans (15) and C. glabrata (16) isolates, alone or mixed, were identified correctly using both protocols, whereas 18 isolates (five other species) were negative by both protocols. This shortened method will significantly reduce the time to identification.

* Corresponding author. Mailing address: Microbiology Laboratory, Meyer B1-193, Department of Pathology, the Johns Hopkins Hospital, 600 N. Wolfe Street, Baltimore, MD 21287. Phone: (410) 955-5077. Fax: (410) 614-8780. E-mail: wmerz{at}jhmi.edu

{triangledown} Published ahead of print on 5 November 2008.

Journal of Clinical Microbiology, January 2009, p. 247-248, Vol. 47, No. 1
0095-1137/09/$08.00+0     doi:10.1128/JCM.01241-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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