This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sako, Y.
Right arrow Articles by Ito, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sako, Y.
Right arrow Articles by Ito, A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, January 2009, p. 252-254, Vol. 47, No. 1
0095-1137/09/$08.00+0     doi:10.1128/JCM.01476-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Development of an Immunochromatographic Test To Detect Antibodies against Recombinant Em18 for Diagnosis of Alveolar Echinococcosis{triangledown}

Yasuhito Sako,1* Kenta Fukuda,2 Yukuharu Kobayashi,2 and Akira Ito1

Department of Parasitology, Asahikawa Medical College, Midorigaoka Higashi 2-1-1-1, Asahikawa, 078-8510 Hokkaido, Japan,1 Division of Research and Development, Adtec Co., Ltd., Yokkaichi 1693-6, Usa, 879-0471 Oita, Japan2

Received 1 August 2008/ Returned for modification 17 September 2008/ Accepted 29 October 2008

An immunochromatographic test (ICT) for the rapid detection of antibodies to Echinococcus multilocularis was developed. The ICT showed a sensitivity of 94% and a specificity of 95.4%. High degrees of agreement were observed between the ICT and an enzyme-linked immunosorbent assay ({kappa} = 0.93) and between the ICT and immunoblot analysis ({kappa} = 0.97). It is expected that the ICT developed in this study will be useful for the serodiagnosis of alveolar echinococcosis.

* Corresponding author. Mailing address: Department of Parasitology, Asahikawa Medical College, Midorigaoka Higashi 2-1, Asahikawa, 078-8510 Hokkaido, Japan. Phone: 81-166-68-2422. Fax: 81-166-68-2429. E-mail: yasusako{at}asahikawa-med.ac.jp

{triangledown} Published ahead of print on 5 November 2008.

Journal of Clinical Microbiology, January 2009, p. 252-254, Vol. 47, No. 1
0095-1137/09/$08.00+0     doi:10.1128/JCM.01476-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ishikawa, Y., Sako, Y., Itoh, S., Ohtake, T., Kohgo, Y., Matsuno, T., Ohsaki, Y., Miyokawa, N., Nakao, M., Nakaya, K., Ito, A. (2009). Serological Monitoring of Progression of Alveolar Echinococcosis with Multiorgan Involvement by Use of Recombinant Em18. J. Clin. Microbiol. 47: 3191-3196 [Abstract] [Full Text]  
  • Kishimoto, M., Yamada, K., Yamano, K., Kobayashi, N., Fujimoto, S., Shimizu, J., Lee, K.-J., Iwasaki, T., Miyake, Y.-I. (2009). Significance of Imaging Features of Alveolar Echinococcosis in Studies on Nonhuman Primates. Am J Trop Med Hyg 81: 540-544 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»