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Journal of Clinical Microbiology, February 2009, p. 441-444, Vol. 47, No. 2
0095-1137/09/$08.00+0     doi:10.1128/JCM.01989-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Comparison of Commercial and In-House Real-Time PCR Assays Used for Detection of Mycoplasma pneumoniae{triangledown}

Roger Dumke* and Enno Jacobs

Technical University Dresden, Medical Faculty Carl Gustav Carus, Institute of Medical Microbiology and Hygiene, Fetscherstrasse 74, D-01307 Dresden, Germany

Received 15 October 2008/ Returned for modification 20 November 2008/ Accepted 5 December 2008

We tested two commercial and three in-house PCR assays under standardized conditions to detect Mycoplasma pneumoniae. All five procedures were able to demonstrate M. pneumoniae DNA in a concentration comparable to 1 CFU/µl, but the mean crossing points resulted in differences in the concentration of the genome copies of a factor of 20.

* Corresponding author. Mailing address: Technical University Dresden, Medical Faculty Carl Gustav Carus, Institute of Medical Microbiology and Hygiene, Fetscherstrasse 74, D-01307 Dresden, Germany. Phone: 49 351 4586577. Fax: 49 351 4586310. E-mail: roger.dumke{at}tu-dresden.de

{triangledown} Published ahead of print on 10 December 2008.

Journal of Clinical Microbiology, February 2009, p. 441-444, Vol. 47, No. 2
0095-1137/09/$08.00+0     doi:10.1128/JCM.01989-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Touati, A., Benard, A., Hassen, A. B., Bebear, C. M., Pereyre, S. (2009). Evaluation of Five Commercial Real-Time PCR Assays for Detection of Mycoplasma pneumoniae in Respiratory Tract Specimens. J. Clin. Microbiol. 47: 2269-2271 [Abstract] [Full Text]  


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