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Journal of Clinical Microbiology, March 2009, p. 674-679, Vol. 47, No. 3
0095-1137/09/$08.00+0     doi:10.1128/JCM.01028-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Simple, Sensitive, and Swift Sequencing of Complete H5N1 Avian Influenza Virus Genomes{triangledown} ,{dagger}

Dirk Höper, Bernd Hoffmann, and Martin Beer*

Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Südufer 10, Greifswald-Insel Riems 17493, Germany

Received 29 May 2008/ Returned for modification 2 September 2008/ Accepted 14 December 2008

The spread of highly pathogenic avian influenza A virus (HPAIV) of subtype H5N1 demands fast and reliable methods for in-depth, full-length sequence analysis. For this purpose, we designed a simple and sensitive method for the preparation of sequencing libraries from H5N1 HPAIV diagnostic RNA samples for sequencing with the Genome Sequencer FLX instrument. The method presented seamlessly integrates high-throughput pyrosequencing with the Roche/454 instrument into diagnostics without the need for additional equipment or molecular biological techniques besides standard PCR and the Genome Sequencer FLX sample preparation and sequencing pipeline.

* Corresponding author. Mailing address: Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, Südufer 10, Greifswald-Insel Riems 17493, Germany. Phone: 49 38351 7200. Fax: 49 38351 7151. E-mail: Martin.Beer{at}fli.bund.de

{triangledown} Published ahead of print on 24 December 2008.

{dagger} This paper is dedicated to the memory of Daniela Hase.

Journal of Clinical Microbiology, March 2009, p. 674-679, Vol. 47, No. 3
0095-1137/09/$08.00+0     doi:10.1128/JCM.01028-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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