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Journal of Clinical Microbiology, March 2009, p. 689-696, Vol. 47, No. 3
0095-1137/09/$08.00+0     doi:10.1128/JCM.01914-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Association of Unique, Isolated Treponemes with Bovine Digital Dermatitis Lesions{triangledown}

Nicholas J. Evans,1,2* Jennifer M. Brown,1 Ibrahim Demirkan,1,{dagger} Prem Singh,2,3 Brian Getty,4 Dorina Timofte,2 W. Daan Vink,2 Richard D. Murray,2 Roger W. Blowey,5 Richard J. Birtles,1 C. Anthony Hart,4 and Stuart D. Carter1,2

Department of Veterinary Pathology, Faculty of Veterinary Science, University of Liverpool, Liverpool L69 3BX, United Kingdom,1 Department of Veterinary Clinical Science, Faculty of Veterinary Science, University of Liverpool, Leahurst, Neston CH64 7TE, United Kingdom,2 Department of Veterinary Surgery and Radiology, CCS Haryana Agricultural University, Hisar 125004, Haryana, India,3 Department of Medical Microbiology, Duncan Building, University of Liverpool, Liverpool L69 3GA, United Kingdom,4 Wood Veterinary Group, St. Oswald's Road, Gloucester GL1 2SJ, United Kingdom5

Received 3 October 2008/ Returned for modification 24 November 2008/ Accepted 3 January 2009

This study used a PCR-based approach targeting 16S rRNA gene fragments to determine the occurrence and association of the three bovine digital dermatitis (BDD) treponeme phylogroups within lesions found in cattle from the United Kingdom. Examination of 51 BDD lesions collected from infected cattle across the United Kingdom revealed that BDD treponeme group 1 (Treponema medium/Treponema vincentii-like), group 2 (Treponema phagedenis-like), and group 3 (Treponema putidum/Treponema denticola-like) were present in 96.1%, 98%, and 76.5% of BDD lesions, respectively. The three phylogroups were present together in 74.5% of lesions. The PCR assays enabled the isolation of further treponeme strains from previously mixed primary BDD lesion cultures. Here a representative from each of the three distinct treponeme phylogroups was isolated from a single BDD lesion for the first time. These data highlight the extent to which this disease is polytreponemal. Immunohistochemistry and electron microscopy were used to investigate lesional hoof tissues, resulting in treponemes being identified copiously in hair follicles and sebaceous glands, suggesting a potential route of exit and/or entry for these pathogens. This study gives further evidence for the importance of the three treponeme groups in BDD pathogenesis and reiterates the value of molecular genetic approaches for isolating and identifying fastidious anaerobes.

* Corresponding author. Mailing address: Department of Veterinary Pathology, Faculty of Veterinary Science, University of Liverpool, Liverpool L69 3BX, United Kingdom. Phone: 44 151 794 4209. Fax: 44 151 794 4219. E-mail: evansnj{at}liverpool.ac.uk

{triangledown} Published ahead of print on 14 January 2009.

{dagger} Present address: Department of Surgery, Faculty of Veterinary Medicine, Afyon Kocatepe University, 03200 Afyonkarahisar, Turkey.

Journal of Clinical Microbiology, March 2009, p. 689-696, Vol. 47, No. 3
0095-1137/09/$08.00+0     doi:10.1128/JCM.01914-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sayers, G., Marques, P. X., Evans, N. J., O'Grady, L., Doherty, M. L., Carter, S. D., Nally, J. E. (2009). Identification of Spirochetes Associated with Contagious Ovine Digital Dermatitis. J. Clin. Microbiol. 47: 1199-1201 [Abstract] [Full Text]  


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