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Journal of Clinical Microbiology, March 2009, p. 765-770, Vol. 47, No. 3
0095-1137/09/$08.00+0     doi:10.1128/JCM.01721-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Simultaneous Detection and Quantitation of Cytomegalovirus, Epstein-Barr Virus, and Adenovirus by Use of Real-Time PCR and Pooled Standards{triangledown}

R. N. Gunson,* A. R. Maclean, S. J. Shepherd, and W. F. Carman

West Of Scotland Specialist Virology Centre, a Member of the UK Clinical Virology Network, Gartnavel General Hospital, Great Western Road, Glasgow, G12 OYN, Scotland

Received 5 September 2008/ Returned for modification 16 October 2008/ Accepted 30 December 2008

Quantitative real-time PCR has become the most widely used preemptive approach for managing cytomegalovirus (CMV), Epstein-Barr virus (EBV), and adenovirus infections in immunosuppressed patients. These three assays are normally available as separate tests, each using five quantitation standards that are tested in duplicate. We have developed an adenovirus-CMV-EBV triplex assay that uses one set of five pooled quantitative standards, tested singly rather than in duplicate. This test demonstrated a sensitivity and an accuracy of quantitation equivalent to those of our previous single tests and was shown to be able to detect mixed infections with no loss in sensitivity. This assay is now in routine use in our laboratory and has considerably simplified the work flow of the laboratory, with a resultant improvement in sample turnaround time and significantly reduced costs.

* Corresponding author. Mailing address: West Of Scotland Specialist Virology Centre, Gartnavel General Hospital, Great Western Road, Glasgow, G12 OYN, Scotland. Phone: 44-141-211-0080. Fax: 44-141-211-0082. E-mail: rory.gunson{at}northglasgow.scot.nhs.uk

{triangledown} Published ahead of print on 14 January 2009.

Journal of Clinical Microbiology, March 2009, p. 765-770, Vol. 47, No. 3
0095-1137/09/$08.00+0     doi:10.1128/JCM.01721-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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