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Journal of Clinical Microbiology, March 2009, p. 807-810, Vol. 47, No. 3
0095-1137/09/$08.00+0     doi:10.1128/JCM.01324-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Detection and Toxin Typing of Clostridium perfringens in Formalin-Fixed, Paraffin-Embedded Tissue Samples by PCR{triangledown}

Josephine Wu,1 Wandi Zhang,1 Boxun Xie,1 Maoxin Wu,1 Xiaodi Tong,1 Jayant Kalpoe,1,2 and David Zhang1*

Department of Pathology, Mount Sinai School of Medicine, One Gustave L. Levy Place, Box 1122, New York, New York 10029,1 Department of Medical Microbiology, Leiden University Medical Center, Albinusdreef 2, 2300RC Leiden, The Netherlands2

Received 11 July 2008/ Returned for modification 25 August 2008/ Accepted 12 December 2008

Since current microbiology methods are not suitable to detect Clostridium perfringens in formalin-fixed, paraffin-embedded tissue samples, we developed a PCR assay to detect toxin-encoding genes and the 16S rRNA gene of C. perfringens. We successfully detected and genotyped C. perfringens in tissue sections from two autopsy cases.

* Corresponding author. Mailing address: Department of Pathology, Mount Sinai School of Medicine, One Gustave L. Levy Place, Box 1122, New York, NY 10029. Phone: (212) 659-8173. Fax: (212) 427-2082. E-mail: david.zhang{at}mssm.edu

{triangledown} Published ahead of print on 24 December 2008.

Journal of Clinical Microbiology, March 2009, p. 807-810, Vol. 47, No. 3
0095-1137/09/$08.00+0     doi:10.1128/JCM.01324-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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