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Journal of Clinical Microbiology, March 2009, p. 830-832, Vol. 47, No. 3
0095-1137/09/$08.00+0     doi:10.1128/JCM.01724-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Utility of Peptide Nucleic Acid Fluorescence In Situ Hybridization for Rapid Detection of Acinetobacter spp. and Pseudomonas aeruginosa{triangledown}

A. Y. Peleg,1,2* Y. Tilahun,3 M. J. Fiandaca,3 E. M. C. D'Agata,1,2 L. Venkataraman,1 R. C. Moellering Jr.,1,2 and G. M. Eliopoulos1,2

Beth Israel Deaconess Medical Center,1 Harvard Medical School, Boston, Massachusetts,2 AdvanDx, Inc., Woburn, Massachusetts3

Received 5 September 2008/ Returned for modification 7 October 2008/ Accepted 22 December 2008

The utility of peptide nucleic acid fluorescence in situ hybridization (PNA FISH) for the detection of Acinetobacter spp. and Pseudomonas aeruginosa was evaluated on broth suspensions and spiked blood cultures of ATCC strains and clinical isolates with select gram-negative rods. After testing 60 clinical isolates, PNA FISH had a sensitivity and specificity of 100% and 100%, respectively, for Acinetobacter spp. and 100% and 95%, respectively, for P. aeruginosa. PNA FISH was able to detect both pathogens simultaneously and directly from spiked blood cultures.

* Corresponding author. Mailing address: Division of Infectious Diseases, Beth Israel Deaconess Medical Center, Boston, MA 02215. Phone: (617) 632-0233. Fax: (617) 632-7626. E-mail: apeleg{at}bidmc.harvard.edu

{triangledown} Published ahead of print on 30 December 2008.

Journal of Clinical Microbiology, March 2009, p. 830-832, Vol. 47, No. 3
0095-1137/09/$08.00+0     doi:10.1128/JCM.01724-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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