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Journal of Clinical Microbiology, April 2009, p. 1096-1100, Vol. 47, No. 4
0095-1137/09/$08.00+0     doi:10.1128/JCM.01899-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Development and Validation of a Microsphere-Based Luminex Assay for Rapid Identification of Clinically Relevant Aspergilli{triangledown}

Kizee A. Etienne,1 Rui Kano,1,2 and S. Arunmozhi Balajee1*

Mycotic Disease Branch, Centers for Disease Control and Prevention, Atlanta, Georgia,1 Department of Pathobiology, Nihon University School of Veterinary Medicine, 1866 Kameino, Fuzisawa-city, Kanagawa 252-8510, Japan2

Received 1 October 2008/ Returned for modification 23 January 2009/ Accepted 13 February 2009

A Luminex-based assay for the rapid identification of Aspergillus species was designed, optimized, and validated with 131 clinical isolates of Aspergillus fumigatus, A. flavus, A. niger, A. terreus, A. ustus, and A. versicolor. The six species-specific probes were directed toward the internal transcribed spacer 1 (ITS-1) region and tested in a multiplex format with results generated within 6 h. Species identifications generated by the Aspergillus Luminex assay were 100% concordant with results from comparative sequence analyses of the ITS-1 region and showed excellent specificity. The Aspergillus Luminex assay is a rapid, relatively simple method that may prove to be a useful diagnostic tool for rapid Aspergillus identification in clinical laboratory settings.

* Corresponding author. Mailing address: Mycotic Diseases Branch, Centers for Disease Control and Prevention, Mail Stop-G 11, 1600 Clifton Road, Atlanta, GA 30333. Phone: (404) 639-3337. Fax: (404) 639-3546. E-mail: fir3{at}cdc.gov

{triangledown} Published ahead of print on 25 February 2008.

Journal of Clinical Microbiology, April 2009, p. 1096-1100, Vol. 47, No. 4
0095-1137/09/$08.00+0     doi:10.1128/JCM.01899-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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