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Journal of Clinical Microbiology, April 2009, p. 1206-1208, Vol. 47, No. 4
0095-1137/09/$08.00+0     doi:10.1128/JCM.02097-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Evaluation of a Rapid Fluorescent Staining Method for Detection of Mycobacteria in Clinical Specimens {triangledown}

Cindy Hendry, Kim Dionne, Annie Hedgepeth, Karen Carroll, and Nicole Parrish*

The Johns Hopkins Medical Institutions, Baltimore, Maryland

Received 31 October 2008/ Returned for modification 22 December 2008/ Accepted 13 February 2009

Rapid detection of mycobacterial disease is essential. Using multiple specimen types and concentrations of mycobacteria, we compared two commercial auramine O stains. The more rapid stain permitted consistent acid-fast bacillus quantitation and exhibited less debris staining, and the staining procedure required less time (~2 min) to perform. These results suggest that the rapid stain may be more cost-effective and efficient for use in clinical laboratories.

* Corresponding author. Mailing address: Johns Hopkins University, Microbiology Division, Meyer B1-193, 600 N. Wolfe St., Baltimore, MD 21287. Phone: (410) 955-5077. Fax: (410) 614-8087. E-mail: nparrish{at}jhmi.edu

{triangledown} Published ahead of print on 25 February 2009.

Journal of Clinical Microbiology, April 2009, p. 1206-1208, Vol. 47, No. 4
0095-1137/09/$08.00+0     doi:10.1128/JCM.02097-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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