This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rodas, C.
Right arrow Articles by Sjöling, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rodas, C.
Right arrow Articles by Sjöling, A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, April 2009, p. 1218-1220, Vol. 47, No. 4
0095-1137/09/$08.00+0     doi:10.1128/JCM.00316-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Development of Multiplex PCR Assays for Detection of Enterotoxigenic Escherichia coli Colonization Factors and Toxins{triangledown}

Claudia Rodas,1,2* Volga Iniguez,2 Firdausi Qadri,3 Gudrun Wiklund,1 Ann-Mari Svennerholm,1 and Åsa Sjöling1

WHO Collaborating Centre for Research on Enterotoxigenic Escherichia coli (ETEC) and Department of Microbiology and Immunology, Institute of Biomedicine, University of Gothenburg, Box 435, 405 30 Göteborg, Sweden,1 Instituto de Biología Molecular y Biotecnología, Universidad Mayor de San Andrés, Facultad de Ciencias Puras y Naturales, Campus Universitario Cota-Cota, Calle 27 s/n La Paz, Bolivia,2 International Centre for Diarrhoeal Disease Research, Bangladesh, G.P.O. Box 128, Dhaka 1000, Bangladesh3

Received 12 February 2009/ Accepted 13 February 2009

Four multiplex PCR assays for detection of 19 enterotoxigenic Escherichia coli (ETEC) colonization factors and an improved ETEC toxin multiplex PCR were developed and tested on Bangladeshi and Bolivian ETEC strain collections. The assays will be useful for surveillance of ETEC infections in diagnostic laboratories that have access to PCR.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Institute of Biomedicine, University of Gothenburg, Box 435, 405 30 Göteborg, Sweden. Phone: 46-31-7866233. Fax: 46-31-7866205. E-mail: claudia.rodas{at}microbio.gu.se

{triangledown} Published ahead of print on 25 February 2009.

Journal of Clinical Microbiology, April 2009, p. 1218-1220, Vol. 47, No. 4
0095-1137/09/$08.00+0     doi:10.1128/JCM.00316-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»