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Journal of Clinical Microbiology, April 2009, p. 924-926, Vol. 47, No. 4
0095-1137/09/$08.00+0     doi:10.1128/JCM.01842-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Sensitivity of PCR Targeting Mycobacterium ulcerans by Use of Fine-Needle Aspirates for Diagnosis of Buruli Ulcer{triangledown}

R. O. Phillips,1,2 F. S. Sarfo,2 F. Osei-Sarpong,3 A. Boateng,3 I. Tetteh,2 A. Lartey,2 E. Adentwe,3 W. Opare,4 K. B. Asiedu,5 and M. Wansbrough-Jones6*

Kwame Nkrumah University of Science and Technology, Kumasi, Ghana,1 Komfo Anokye Teaching Hospital, Kumasi, Ghana,2 Tepa Government Hospital, Tepa, Ghana,3 National Buruli Ulcer Control Programme, Accra, Ghana,4 World Health Organization, Geneva, Switzerland,5 St. George's, University of London, London, United Kingdom6

Received 23 September 2008/ Returned for modification 11 November 2008/ Accepted 30 January 2009

In a previous study, we reported that the sensitivity of PCR targeting the IS2404 insertion sequence of Mycobacterium ulcerans was 98% when it was applied to 4-mm punch biopsy samples of Buruli lesions. Fine-needle aspiration (FNA) is a less traumatic sampling technique for nonulcerated lesions, and we have studied the sensitivity of PCR using FNA samples. Fine-needle aspirates were taken with a 21-gauge needle from 43 patients diagnosed clinically with M. ulcerans disease. Four-millimeter punch biopsies were obtained for microscopy, culture, and PCR targeting the IS2404 insertion sequence. The sensitivity of PCR using samples obtained by FNA was 86% (95% confidence interval [95% CI], 72 to 94%) compared with that for PCR using punch biopsy samples. In this study, the sensitivities of culture and microscopy for punch biopsy samples were 44% (95% CI, 29 to 60%) and 26% (95% CI, 14 to 41%), respectively. This demonstrates that PCR on an FNA sample is a viable minimally invasive technique to diagnose M. ulcerans lesions.

* Corresponding author. Mailing address: St. George's Hospital and Medical School, University of London, London SW17 0RE, United Kingdom. Phone: 44-2087255827. Fax: 44-2087253487. E-mail: wansbrou{at}sghms.ac.uk

{triangledown} Published ahead of print on 9 February 2009.

Journal of Clinical Microbiology, April 2009, p. 924-926, Vol. 47, No. 4
0095-1137/09/$08.00+0     doi:10.1128/JCM.01842-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Eddyani, M., Fraga, A. G., Schmitt, F., Uwizeye, C., Fissette, K., Johnson, C., Aguiar, J., Sopoh, G., Barogui, Y., Meyers, W. M., Pedrosa, J., Portaels, F. (2009). Fine-Needle Aspiration, an Efficient Sampling Technique for Bacteriological Diagnosis of Nonulcerative Buruli Ulcer. J. Clin. Microbiol. 47: 1700-1704 [Abstract] [Full Text]  


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