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Journal of Clinical Microbiology, April 2009, p. 927-931, Vol. 47, No. 4
0095-1137/09/$08.00+0     doi:10.1128/JCM.01564-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Highly Sensitive Detection of Dengue Virus Nucleic Acid in Samples from Clinically Ill Patients{triangledown}

Jorge L. Muñoz-Jordán,1* Cynthia S. Collins,2 Edgardo Vergne,1 Gilberto A. Santiago,1 Lyle Petersen,3 Wellington Sun,1 and Jeffrey M. Linnen2

Gen-Probe Incorporated, San Diego, California,2 Dengue Branch, Division of Vector-Borne Infectious Disease, Centers for Disease Control and Prevention, San Juan, Puerto Rico,1 Division of Vector-Borne Infectious Disease, Centers for Disease Control and Prevention, Fort Collins, Colorado3

Received 12 August 2008/ Returned for modification 1 November 2008/ Accepted 9 February 2009

Dengue virus (DENV) is a major cause of febrile illness and hemorrhagic fever in tropical and subtropical regions. Typically, patients presenting with acute dengue disease are viremic but may not have yet developed detectable titers of antibody. Therefore, early diagnosis depends mostly on detection of viral components, such as the RNA. To define the potential use of transcription-mediated amplification (TMA) DENV RNA as a diagnostic tool, we first compared its analytic sensitivity using a routine real-time reverse transcription (RT)-PCR and found that TMA is approximately 10 to 100 times more sensitive. In addition, we tested acute-phase serum samples (<5 days post-symptom onset) submitted as part of laboratory-based surveillance in Puerto Rico and determined that among patients with serologically confirmed dengue infection, TMA detected DENV RNA in almost 80% of serum specimens that were negative by the RT-PCR test used for diagnosis and in all specimens with positive RT-PCR results. We conclude that TMA is a highly sensitive method which can detect DENV RNA in approximately 89% of clinical, acute-phase serum specimens.

* Corresponding author. Mailing address: Molecular Diagnostics and Research Laboratory, Centers for Disease Control and Prevention, 1324 Calle Canada, San Juan, PR 00920-3860. Phone: 787-7062399. Fax: 787-7062496. E-mail: ckq2{at}cdc.gov

{triangledown} Published ahead of print on 18 February 2009.

Journal of Clinical Microbiology, April 2009, p. 927-931, Vol. 47, No. 4
0095-1137/09/$08.00+0     doi:10.1128/JCM.01564-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Arya, S. C., Agarwal, N., Munoz-Jordan, J. L. (2009). NS1 Detection in Addition to Reverse Transcriptase PCR and Transcription-Mediated Amplification of Dengue Virus RNA in Acutely Ill Patients. J. Clin. Microbiol. 47: 1983-1983 [Full Text]  


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