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Journal of Clinical Microbiology, May 2009, p. 1578-1580, Vol. 47, No. 5
0095-1137/09/$08.00+0     doi:10.1128/JCM.02507-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Comparison of Routine Bench and Molecular Diagnostic Methods in Identification of Burkholderia pseudomallei{triangledown}

Kelly Hodgson,1 Cathy Engler,2 Brenda Govan,1 Natkunam Ketheesan,1 and Robert Norton2*

School of Veterinary & Biomedical Sciences, James Cook University, Townsville, Queensland, Australia 4814,1 Microbiology, Pathology Queensland, Townsville Hospital, Townsville, Queensland, Australia 48142

Received 31 December 2008/ Returned for modification 14 February 2009/ Accepted 28 February 2009

This study compared the identification of Burkholderia pseudomallei with that of related organisms. Bench tests and latex agglutination were compared with molecular identification. Using bench tests and latex agglutination alone, 100% (30/30) of B. pseudomallei isolates were correctly identified. Amoxicillin-clavulanate susceptibility testing was also a good and simple discriminatory test.


* Corresponding author. Mailing address: Microbiology, Pathology Queensland, The Townsville Hospital, Townsville, Qld 4814, Australia. Phone: 61 (07) 47 961111. Fax: 61 (07) 47 962415. E-mail: Robert_Norton{at}health.qld.gov.au

{triangledown} Published ahead of print on 11 March 2009.


Journal of Clinical Microbiology, May 2009, p. 1578-1580, Vol. 47, No. 5
0095-1137/09/$08.00+0     doi:10.1128/JCM.02507-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.