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Journal of Clinical Microbiology, June 2009, p. 1689-1694, Vol. 47, No. 6
0095-1137/09/$08.00+0     doi:10.1128/JCM.02179-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Evaluation of the BD GeneOhm StaphSR Assay for Detection of Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus Isolates from Spiked Positive Blood Culture Bottles {triangledown}

Sabine Gröbner,1 Mireille Dion,2 Mélanie Plante,2 and Volkhard A. J. Kempf3*

Institute of Medical Microbiology and Hygiene, University of Tübingen, Tübingen, Germany,1 BD Diagnostics GeneOhm, Québec, Canada,2 Institute of Medical Microbiology and Infection Control, University of Frankfurt am Main, Frankfurt am Main, Germany3

Received 13 November 2008/ Returned for modification 30 January 2009/ Accepted 3 April 2009

To improve the clinical outcome of Staphylococcus aureus septicemia, the early selection of appropriate antibiotic treatment is crucial. Molecular diagnostics represents an attractive approach for the rapid identification of S. aureus and the determination of its methicillin (meticillin) resistance. In direct comparison to other molecular assays (sa442 and mecA real-time PCRs) and standard laboratory procedures, we evaluated the BD GeneOhm StaphSR assay for its use in the detection of S. aureus and methicillin-resistant S. aureus (MRSA) from spiked blood culture bottles (n = 134). In the case of detecting S. aureus (n = 90; for methicillin-susceptible S. aureus, n = 45; for MRSA, n = 45), the BD GeneOhm StaphSR assay had a sensitivity and a specificity of 100% each (95% confidence intervals [CIs], 96.0 to 100% and 82.4 to 100%, respectively). For MRSA (n = 45), the test was 95.6% (95% CI, 84.9 to 99.5%) sensitive and 95.3% (95% CI, 86.9 to 99.0%) specific. Overall, five discrepant results arose with this assay due to the presence of methicillin-susceptible, revertant MRSA strains (3/45) and MRSA strains that were not detected by the BD GeneOhm StaphSR assay (2/45). Compared to other real-time PCR-based molecular approaches and to conventional standard laboratory methods, the BD GeneOhm StaphSR turned out to be an appropriate diagnostic tool for a rapid (~1.5 h), preliminary identification of S. aureus and MRSA from blood cultures.

* Corresponding author. Mailing address: Institut für Medizinische Mikrobiologie und Krankenhaushygiene, Klinikum der Johann Wolfgang Goethe-Universität, Paul Ehrlich-Str. 40, D-60596 Frankfurt am Main, Germany. Phone: 49-69-6301-5019. Fax: 49-69-6301-83438. E-mail: volkhard.kempf{at}kgu.de

{triangledown} Published ahead of print on 15 April 2009.

Journal of Clinical Microbiology, June 2009, p. 1689-1694, Vol. 47, No. 6
0095-1137/09/$08.00+0     doi:10.1128/JCM.02179-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Snyder, J. W., Munier, G. K., Heckman, S. A., Camp, P., Overman, T. L. (2009). Failure of the BD GeneOhm StaphSR Assay for Direct Detection of Methicillin-Resistant and Methicillin-Susceptible Staphylococcus aureus Isolates in Positive Blood Cultures Collected in the United States. J. Clin. Microbiol. 47: 3747-3748 [Abstract] [Full Text]  


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