This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Google Scholar
Right arrow Articles by Lartigue, M.-F.
Right arrow Articles by Quentin, R.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lartigue, M.-F.
Right arrow Articles by Quentin, R.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, July 2009, p. 2284-2287, Vol. 47, No. 7
0095-1137/09/$08.00+0     doi:10.1128/JCM.00175-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Identification of Streptococcus agalactiae Isolates from Various Phylogenetic Lineages by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry{triangledown}

Marie-Frédérique Lartigue,1,2* Geneviève Héry-Arnaud,1 Eve Haguenoer,1 Anne-Sophie Domelier,1,2 Pierre-Olivier Schmit,3 Nathalie van der Mee-Marquet,1,2 Philippe Lanotte,1 Laurent Mereghetti,1 Markus Kostrzewa,4 and Roland Quentin1,2

Université François-Rabelais de Tours, UFR de Médecine, EA 3854 Bactéries et risque materno-foetal, Institut Fédératif de Recherche 136 Agents Transmissibles et Infectiologie, 37032 Tours Cedex, France,1 Service de Bactériologie et Hygiène Hospitalière, Hôpital Trousseau, CHRU de Tours, 37044 Tours, France,2 Bruker Daltonique, 34 rue de l'Industrie, 67166 Wissembourg Cedex, France,3 Bruker Daltonik, GmbH, Permoser Str. 15, D-04318 Leipzig, Germany4

Received 28 January 2009/ Returned for modification 9 March 2009/ Accepted 24 April 2009

Variations in proteins related to bacterial diversity may affect species identification performed using matrix-assisted laser desorption ionization (MALDI)-time of flight mass spectrometry. Using this method, we identified 110 Streptococcus agalactiae isolates characterized by serotyping and multilocus sequence typing. Serotype III and sequence type 23 strains expressed the widest variation in molecular weight of putative "species-identifying" biomarker ions. Recognition of the diversity of MALDI patterns observed in strains that represent all major intraspecies lineages assists in the constitution of an optimal reference database.

* Corresponding author. Mailing address: Marie-Frédérique Lartigue, Service de Bactériologie-Hygiène, CHRU Trousseau, 37044 Tours Cedex 9, France. Phone: 33 2 47 47 81 13. Fax: 33 2 47 47 85 30. E-mail: lartigue{at}med.univ-tours.fr

{triangledown} Published ahead of print on 29 April 2009.

Journal of Clinical Microbiology, July 2009, p. 2284-2287, Vol. 47, No. 7
0095-1137/09/$08.00+0     doi:10.1128/JCM.00175-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»