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JCM Accepts, published online ahead of print on 26 March 2008
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J. Clin. Microbiol. doi:10.1128/JCM.00109-08
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

A PCR Method to Identify Salmonella enterica serovars Typhi, Paratyphi A and Paratyphi B Among Salmonella Isolates from the Blood of Patients with Clinical Enteric Fever

Haim Levy, Souleymane Diallo, Sharon M. Tennant, Sofie Livio, Samba O. Sow, Milagritos Tapia, Patricia I. Fields, Matthew Mikoleit, Boubou Tamboura, Karen L. Kotloff, Rosanna Lagos, James P. Nataro, James E. Galen, and Myron M. Levine*

Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, MD 21044; Israel Institute for Biological Research, Ness Ziona, Israel; Centre pour le Développement des Vaccins (CVD-Mali), Bamako, Mali; National Salmonella Reference Laboratory, Centers for Disease Control and Prevention, Atlanta, GA 30333; Centro para Vacunas en Desarrollo, Hospital de Niños Roberto del Rio, Servicio de Salud Metropolitano Norte, Santiago, Chile

* To whom correspondence should be addressed. Email: mlevine{at}medicine.umaryland.edu.


  Abstract

Polymerase chain reaction (PCR) methodology was developed to identify Salmonella enterica serovars Typhi, Paratyphi A and Paratyphi B. One multiplex PCR identifies serogroup D, A and B and Vi-positive strains; another confirms flagellar antigens "d", "a", or "b". Blinded testing of 664 Malian and Chilean Salmonella blood isolates, demonstrated 100% sensitivity and specificity.




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