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J. Clin. Microbiol. doi:10.1128/JCM.00139-08
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Evaluation of 16S rRNA gene-based PCR assays for genus-level identification of Helicobacter species

Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium

^* To whom correspondence should be addressed. Email: Hilde.Moyaert{at}UGent.be.

	Abstract

The inclusivity, exclusivity and detection limit of six 16S rRNA gene-based Helicobacter genus-specific PCR assays was examined. Five out of six assays were 100% inclusive, but the tests varied considerably in their exclusivity (9.1% to 95.5%). The clinical detection limit varied between 10³ and 1 viable bacterial cell per reaction mixture.