This Article Full Text (PDF) Other Versions of this Article: JCM.00305-07v1 45/8/2480    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Klungthong, C. Articles by Jarman, R. G. Search for Related Content PubMed PubMed Citation Articles by Klungthong, C. Articles by Jarman, R. G.

 Previous Article  |  Next Article 

J. Clin. Microbiol. doi:10.1128/JCM.00305-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Dengue Viral Detection using Whole Blood for RT-PCR and Viral Isolation

Armed Forced Research Institute of Medical Sciences; Department of Virology, Bangkok, Thailand; Queen Sirikit National Institute of Child Health, Bangkok 10400, Thailand

^* To whom correspondence should be addressed. Email: Richard.Jarman{at}afrims.org.

	Abstract

Dengue is one of the most important diseases in the tropical and subtropical world with an estimated 2.5 billion people at risk. Detection of dengue viral infections has great importance for clinical management of patients, surveillance, and clinical trial assessments. Traditionally blood samples are collected in serum separator tubes, processed for serum, and then taken to the lab for analysis. Using whole blood has the potential advantages of requiring less blood, providing quicker results, and perhaps better sensitivity during the acute phase of the disease. We compared RT-PCR results from blood drawn into EDTA tubes and serum separator tubes from 108 clinically suspected dengue infected individuals. We determined that RNA extraction from whole blood followed by RT-PCR resulted in a higher detection rate than using serum or plasma. Using a selection of these samples we also found that our ability to detect virus by direct C6/36 cell culture and mosquito inoculation was enhanced using whole blood, but not to the same extent that was seen in RT-PCR.

This article has been cited by other articles:

• Das, S., Pingle, M. R., Munoz-Jordan, J., Rundell, M. S., Rondini, S., Granger, K., Chang, G.-J. J., Kelly, E., Spier, E. G., Larone, D., Spitzer, E., Barany, F., Golightly, L. M. (2008). Detection and Serotyping of Dengue Virus in Serum Samples by Multiplex Reverse Transcriptase PCR-Ligase Detection Reaction Assay. J. Clin. Microbiol. 46: 3276-3284 [Abstract] [Full Text]