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JCM Accepts, published online ahead of print on 21 March 2007
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JCM.00382-07v1
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J. Clin. Microbiol. doi:10.1128/JCM.00382-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Surveillance for reassortant virus by multiplex RT-PCR specific for eight genomic segments of avian influenza A H5N1 viruses

Prasert Auewarakul, Kantima Sangsiriwut, Kridsda Chaichoune, Arunee Thititanyanont, Witthawat Wiriyarat, Taweesak Songserm, Rasameepen Ponak-nguen, Jarunee Prasertsopon, Phisanu Pooruk, Pathom Sawanpanyalert, Parntep Ratanakorn, and Pilaipan Puthavathana*

Department of Microbiology, Faculty of Medicine Siriraj Hospital; Faculty of Veterinary Science; Faculty of Science, Mahidol University; Faculty of Veterinary Medicine, Kasetsart University; and Department of Medical Sciences, Ministry of Public Health, Thailand

* To whom correspondence should be addressed. Email: siput{at}mahidol.ac.th.


  Abstract

Avian influenza H5N1 virus is a global threat. An emergence of a reassortant virus with a pandemic potential is a major concern. Here we describe a multiplex RT-PCR that is specific for the eight genomic segments of the currently circulating H5N1 viruses to facilitate the surveillance for a reassortant between human influenza virus and the H5N1 virus.




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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
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Copyright © 2007 by the American Society for Microbiology. All rights reserved.