JCM Accepts, published online ahead of print on 11 October 2006
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J. Clin. Microbiol. doi:10.1128/JCM.00421-06
Copyright (c) 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Genotyping of Clinical Herpes Simplex Virus Type 1 Isolates by Restriction Enzymes

Peter Norberg*, Tomas Bergström, and Jan-Åke Liljeqvist

Department of Clinical Virology, Göteborg University, S-413 46 Göteborg, Sweden

* To whom correspondence should be addressed. Email: peter.norberg{at}microbio.gu.se.


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Abstract

Recently three distinct genotypes of clinical herpes simplex virus type 1 (HSV-1) isolates were identified based on DNA sequence information and phylogenetic analysis of clinical isolates as well as laboratory strains. Here we utilized single nucleotide polymorphism within the genes coding for glycoproteins G and I for rapid genotype classification by PCR and restriction enzyme cleavage. The method is suitable for high-scale genotyping of clinical HSV-1 isolates and for detection of recombinants.




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