JCM Accepts, published online ahead of print on 18 June 2008

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J. Clin. Microbiol. doi:10.1128/JCM.00450-08
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Multi-Center Evaluation of the VITEK 2 Anaerobes & Corynebacteria (ANC) Identification Card

Robert P. Rennie^*, Cheryl Brosnikoff, LeeAnn Turnbull, L. Barth Reller, Stanley Mirrett, William Janda, Kathy Ristow, and Ann Krilcich

Medical Microbiology Laboratory, University of Alberta Hospital, Edmonton, AB, CANADA; Clinical Microbiology Laboratory, Duke University Hospital, Durham, NC, USA; University of Illinois Medical Center, Chicago, IL, USA

The new Anaerobic & Corynebacteria (ANC) identification card for VITEK 2 was compared with a 16S rRNA gene sequencing (16S) reference method for accurate identification of corynebacteria and anaerobic species. Testing was performed on the VITEK 2 XL system with modified software at three clinical trial laboratories. Reproducibility was determined with nine ATCC quality control strains tested 20 times over a minimum of 10 days at all three sites. A challenge set of 50 strains with known identifications and 365 recent fresh and frozen clinical isolates were included in the study. Expected positive and negative biochemical well reactions were also evaluated for substrate reproducibility. All strains were tested on the ANC card and clinical isolates were saved for 16S rRNA gene sequencing. All reproducibility tests yielded expected results within a 95% confidence interval except for C. striatum ATCC 6940 which failed at one trial site. For challenge isolates, there was 98% correct identification, 5% low discrimination, 2% incorrect identification and 0% unidentified. For clinical strains, there was 95.1% correct identification, 4.9% low discrimination, 4.6% incorrect identification, and 0.3% were unidentified. The 4.6% (17/365) clinical isolates that gave an incorrect identification consisted of 14 isolates correct to genus and three incorrect to genus. The new ANC card met all performance criteria within a 95% confidence interval when compared to identification by 16S rRNA gene sequencing.

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• Mory, F., Alauzet, C., Matuszeswski, C., Riegel, P., Lozniewski, A. (2009). Evaluation of the New Vitek 2 ANC Card for Identification of Medically Relevant Anaerobic Bacteria. J. Clin. Microbiol. 47: 1923-1926 [Abstract] [Full Text]