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JCM Accepts, published online ahead of print on 25 July 2007
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J. Clin. Microbiol. doi:10.1128/JCM.00458-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Comparison of hand-held test kits, immunofluorescence microscopy, ELISA, and flow cytometric analysis for the rapid presumptive identification of Yersinia pestis

H Tomaso, P Thullier, E Seibold, V Guglielmo, A Buckendahl, L Rahalison, H Neubauer, H C Scholz, and W D Splettstoesser

Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, 80937 Munich, Germany; Groupe de biotechnologie des anticorps, Départment de Biologie des Agents Transmissibles, Centre de Recherches du Service de Santé des Armées, 24 Avenue des Maquis du Grésivaudan, P.O. Box 87, 38702 La Tronche, France; World Health Organization Collaborating Center for Plague, Insitut Pasteur de Madagascar, Route de llnstitut Pasteur, Ambatofotsikely-Avaradoha, P.O. Box 1274, Antananarivo 101, Madagascar.; Friedrich Loeffler Institut, Naumburgerstrasse 96a, 07743 Jena, Germany


  Abstract

An in-house immunochromatographic (ICT) test, the Plague BioThreat AlertTM test strips, ABICAPTM columns, ELISA, flow cytometry and immunofluorescence microscopy were compared for the detection of the F1 capsular antigen of Yersinia pestis, using spiked buffer and clinical specimens. Hand-held test kits proved to be excellent bench top tools.




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