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J. Clin. Microbiol. doi:10.1128/JCM.00684-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Molecular study of microsporidiosis due to E. bieneusi and E. intestinalis among HIV-infected patients from two geographical areas: Niamey (Niger) and Hanoi (Vietnam)

Laboratory of Parasitology and Mycology, Nantes University Hospital, France, National Hospital of Niamey, Niger, Solthis Niger, Institute of Hygiene and Epidemiology of Hanoi, Vietnam, Virology Laboratory, Nantes University Hospital, France

^* To whom correspondence should be addressed. Email: francoise.gay-andrieu{at}chu-nantes.fr.

	Abstract

Microsporidiosis due to Enterocytozoon bieneusi and Encephalitozoon intestinalis are emerging opportunistic infections associated with a wide range of clinical syndromes in humans. The aim of this study was to specify microsporidia epidemiology in two different geographical areas.

From November 2004 to August 2005, 228 and 42 stool samples were respectively collected in Niamey (Niger) and Hanoi (Vietnam). Screening for microsporidia was performed using UV-light microscopy. Diagnosis was confirmed by molecular biology using two specific methods for E. bieneusi and E. intestinalis. All positive samples for E. bieneusi were subjected to genotyping.

In this study, we found a high prevalence of microsporidiosis among HIV-infected patients of 10.5% and 9.5% respectively in Niamey and Hanoi. This level of prevalence is similar to those recorded in European countries before Highly Active Antiretroviral Therapy. From the samples positive for E. bieneusi we found seven distinct genotypes including two genotypes not already described. E. bieneusi genotypes distribution between the two geographical areas suggests different sources of infection, person-to-person in Niger versus zoonotic transmission in Vietnam.

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