JCM
Home Help [Feedback] [For Subscribers] [Archive] [Search] --
JCM Accepts, published online ahead of print on 19 December 2007
This Article
Right arrow Full Text (PDF)
Right arrow Other Versions of this Article:
JCM.00801-07v1
46/2/644    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Blyn, L. B.
Right arrow Articles by Ecker, D. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Blyn, L. B.
Right arrow Articles by Ecker, D. J.

 Previous Article  |  Next Article 

J. Clin. Microbiol. doi:10.1128/JCM.00801-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Rapid Detection and Molecular Serotyping of Adenovirus using PCR followed by Electrospray Ionization Mass Spectrometry

Lawrence B. Blyn*, Thomas A. Hall, Brian Libby, Raymond Ranken, Rangarajan Sampath, Karl Rudnick, Emily Moradi, Anjali Desai, David Metzgar, Kevin L. Russell, Nikki E. Freed, Melinda Balansay, Michael P. Broderick, Miguel A. Osuna, Steven A. Hofstadler, and David J. Ecker

Ibis Biosciences, Inc. Carlsbad, California, and Department of Respiratory Disease Research, Naval Health Research Center, San Diego, California, and Science Applications International Corporation, San Diego, California

* To whom correspondence should be addressed. Email: lblyn{at}isisph.com.


  Abstract

We have developed a PCR/electrospray ionization mass spectrometry (PCR/ESI-MS) assay for the rapid detection, identification, and serotyping of human adenoviruses. The assay employs a high-performance mass spectrometer to "weigh" the amplicons obtained from PCR using primers designed to amplify known human adenoviruses. Masses are converted to base compositions and, by comparison against a database of the genetic sequences, the serotype present in a sample is determined. The performance of the assay was demonstrated with quantified viral standards and environmental and human clinical samples collected from a military training facility. Over 500 samples a day can be analyzed with sensitivities better than 100 genomes per reaction. This approach can be applied to many other families of infectious agents for rapid and sensitive analysis.






Home Help [Feedback] [For Subscribers] [Archive] [Search] --
Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 2007 by the American Society for Microbiology. All rights reserved.