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JCM Accepts, published online ahead of print on 5 September 2007
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J. Clin. Microbiol. doi:10.1128/JCM.00827-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Comparison of Plaque- and Flow Cytometry- Based Methods for Measuring Dengue Virus Neutralization

Annette A. Kraus, William Messer, Laura B. Haymore, and Aravinda M. de Silva*

Department of Microbiology and Immunology, University of North Carolina School of Medicine, Chapel Hill, NC 27599

* To whom correspondence should be addressed. Email: desilva{at}med.unc.edu.


  Abstract

As dengue vaccines enter clinical trials, there is a need for rapid and quantitative assays to measure neutralization. We have developed flow-based neutralization assays, which generated similar results to the established, plaque reduction neutralization test. The flow assays are an improvement as they utilize human cells and allow for high-throughput screening.




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Copyright © 2007 by the American Society for Microbiology. All rights reserved.