JCM Accepts, published online ahead of print on 3 July 2007
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J. Clin. Microbiol. doi:10.1128/JCM.00858-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Development and application of a novel peptide nucleic acid probe for the specific detection of Helicobacter pylori in gastric biopsies

N. GUIMARÃES, N. F. AZEVEDO, C. FIGUEIREDO, C. W. KEEVIL, and M. J. VIEIRA*

IBB - Institute for Biotechnology and Bioengineering, Centre for Biological Engineering, Universidade do Minho, Campus de Gualtar 4710-057, Braga, Portugal; IPATIMUP - Institute of Molecular Pathology and Immunology of the University of Porto, Porto, Portugal; School of Biological Sciences, University of Southampton, UK and Medical Faculty of Porto, Porto, Portugal


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Abstract

In this work, a fluorescence in situ hybridization (FISH) method for the rapid detection of Helicobacter pylori is reported using a novel peptide nucleic acid (PNA) probe. Laboratory testing with several different bacterial species, including other Helicobacter spp., has shown that this probe is highly specific for H. pylori strains. In addition, the PNA FISH method has been successfully adapted for detection of the pathogen in paraffin-embedded gastric biopsies.




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