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J. Clin. Microbiol. doi:10.1128/JCM.00935-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Accuracy of Burkholderia pseudomallei identification using the API 20NE and a latex agglutination test

Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Wellcome Trust-Mahosot Hospital-Oxford Tropical Medicine Research Collaboration, Mahosot Hospital, Vientiane, Lao PDR; Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia; Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam; Melioidosis Research Center, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Nuffield Department of Clinical Medicine, University of Oxford, UK

^* To whom correspondence should be addressed. Email: sharon{at}tropmedres.ac.

	Abstract

In an evaluation of the API 20NE for the identification of Burkholderia spp., 792/800 (99%) B. pseudomallei and 17/19 (89%) B. cepacia isolates were correctly identified, but 10 B. mallei and 98 B. thailandensis isolates were not correctly identified. A latex agglutination test was positive for 796/800 (99.5%) B. pseudomallei isolates and negative for 120 other oxidase positive Gram-negative bacilli.