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J. Clin. Microbiol. doi:10.1128/JCM.01096-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Comparison of MLVA with other PCR-based methods for typing Brucella suis isolates

Departamento de Microbiología y Parasitología, Universidad de Navarra, 31008, Pamplona, Spain, Univ Paris-Sud, Institut de Génétique et Microbiologie, Orsay, F-91405, France; CNRS, Orsay, F-91405, France, Centre d' Etudes du Bouchet 5 rue Lavoisier, 91710 Vert le Petit, France, Centro de Investigación y Tecnología Agroalimentaria de Aragón (CITAA), Gobierno de Aragón, 58080, Zaragoza, Spain, and Croatian Veterinary Institute, Department for Immunology, Savska Cesta 143, 10000, Zagreb, Croatia

^* To whom correspondence should be addressed. Email: ilgoni{at}unav.es.

	Abstract

Multiple-locus variable-number tandem repeat analysis (MLVA), multiplex PCR and PCR-RFLP were compared for typing Brucella suis isolates. A perfect concordance was obtained among these molecular assays. However, MLVA was the only method to demonstrate brucellosis outbreaks and to confirm that wildlife is a reservoir for zoonotic brucellosis.