JCM Accepts, published online ahead of print on 3 January 2008

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J. Clin. Microbiol. doi:10.1128/JCM.01219-07
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Characterization of diarrheagenic Escherichia coli and Shigella strains isolated from children in a hospital case-control study in Hanoi, Vietnam

Bui Thi Thu Hien^*, Flemming Scheutz, Phung Dac Cam, Oralak Serichantalergs, Tran Thu Huong, Tran Minh Thu, and Anders Dalsgaard

Department of Microbiology, National Institute of Hygiene and Epidemiology, Ha noi, Vietnam; The International Escherichia and Klebsiella Centre (WHO), Department of Bacteriology, Mycology, and Parasitology, Statens Serum Institut, Copenhagen, Denmark; Department of Veterinary Pathobiology, Faculty of Life Science, Copenhagen University, Frederiksberg, Denmark; Department of Enteric Pathogens, Armed Forces Research Institute of Medical Science, Bangkok, Thailand

^* To whom correspondence should be addressed. Email: hien.nihe{at}gmail.com.

This case-control study detected and characterized Shigella and diarrheagenic E. coli (DEC) types among Vietnamese children less than five years. In 249 children with diarrhea and 124 controls, Shigella spp. was an important cause of diarrhea (P <0.05). We used multiplex PCR and DNA probes to detect enteroinvasive E. coli (EIEC), enteroaggreative E. coli (EAggEC), enteropathogenic E. coli (EPEC), attaching and effacing E. coli (A/EEC), Verocytotoxin-producing E. coli (VTEC), and enterotoxigenic E. coli (ETEC). The prevalences of DEC in the diarrhea and control groups were 25.7% and 10.5%, respectively. In 62 children with diarrhea, 64 DEC strains included 22 EAggEC (8.8%), 2 EIEC (0.8%), 23 A/EEC (9.2%), 7 EPEC (2.8%) and 10 ETEC strains (4.0%). Among controls, 13 DEC strains included 5 EAggEC (4.0%), 7 A/EEC (5.6%), and 1 EPEC strains. The characterization of DEC by serotypes, antimicrobial susceptibility patterns, virulence genes, and PGFE showed the occurrence of many different and highly heterogenic DEC subtypes, but common serotypes were found among ETEC, EIEC and EPEC, respectively. Serotyping was used to distinguish between A/EEC and EPEC. However, A/EEC, EPEC and EAggEC were isolated at high frequency from both cases and controls. Further in-depth studies are needed to better understand important virulence factors of DEC, especially these A/EEC, EPEC, and EAggEC.

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