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J. Clin. Microbiol. doi:10.1128/JCM.01435-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Colorimetric detection of multi or extensively drug resistant tuberculosis: using Malachite green indicator dye

Mycobacteriology Research Centre, National Research Institute of Tuberculosis and Lung Disease (NRITLD), Shaheed Beheshti university of medical sciences, Darabad, Tehran, 19556, P.O: 19575/154, Iran; Dept of Bacteriology, Swedish Institute for Infectious Disease Control, SE-171 82, Solna, Sweden

^* To whom correspondence should be addressed. Email: pfarnia{at}hotmail.com.

	Abstract

The Malachite green micro tube (MGMT) susceptibility assay was performed directly on sputum specimens (n=80) and indirectly on Mycobacterium tuberculosis (MTB) clinical isolates (n=60).The technique is based on the Malachite green dye, which changes color in response to M.TB growth. The MGMT assay is a simple, rapid and dose not require expensive instruments.

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