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J. Clin. Microbiol. doi:10.1128/JCM.01519-07
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Genotyping of HBV: a comparison of reverse hybridisation, micro array and sequence analysis

Department of Virology, and Gastroenterology, Erasmus MC University Medical Centre Rotterdam, The Netherlands; bioMérieux, Lyon, France

^* To whom correspondence should be addressed. Email: s.pas{at}erasmusmc.nl.

	Abstract

HBV viral genotyping has become important in epidemiological and clinical diagnostic given the relation between the viral genotype and progression of disease or the appearance of antiviral resistance. Since genotyping by sequence and phylogenetic analyses is not convenient in the clinical setting, we evaluated the InnoLipa HBV genotyping (Innogenetics, Belgium) and the prototype HBV DNA-Chip (bioMerieux, France) assays, and compared them to sequencing of the golden standard S gene using a cohort of 275 individual patient samples. All, but 2 samples belonging to distant and individual subgroups within a single genotype, were detected by the InnoLipa HBV assay. Four samples with dual infections belonging to genotype A and G were identified by the InnoLipa HBV assay only. In the HBV DNA-Chip assay, one sample could not be amplified due to a low viral load. Four samples were identified as genotype C and two as genotype D by sequencing but classified as genotype A (2) and D (2), and A(1) and G(1), respectively by the DNA chip assay. In conclusion, the InnoLipa HBV genotyping strip assay detected dual infections and is an easy and quick tool for genotyping, with a sensitivity of 99.3% and a specificity of 100% compared to sequence analysis. HBV DNA-Chip assay showed a sensitivity and specificity of 97.5 and 97.8%, respectively