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JCM Accepts, published online ahead of print on 26 September 2007
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J. Clin. Microbiol. doi:10.1128/JCM.01590-07
Copyright (c) 2007, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Australian and Thai isolates of Burkholderia pseudomallei are distinct by multilocus sequence typing; revision of a case of mistaken identity

Bart J Currie*, Annette D Thomas, Daniel Godoy, David A Dance, Allen C Cheng, Linda Ward, Mark Mayo, Tyrone L Pitt, and Brian G Spratt

Menzies School of Health Research, Charles Darwin University, Darwin Northern Territory Australia; Northern Territory Clinical School, Flinders University, Darwin Northern Territory Australia; Department of Primary Industries and Fisheries, Townsville, Queensland Australia; Department of Infectious Diseases Epidemiology, Faculty of Medicine, Imperial College London, St Mary's Hospital, London, United Kingdom; Health Protection Agency, Plymouth, Devon, United Kingdom; Centre for Infections, Health Protection Agency, London, United Kingdom

* To whom correspondence should be addressed. Email: bart{at}menzies.edu.au.


   Abstract

A recent study using multilocus sequence typing (MLST) of Burkholderia pseudomallei isolates found a sequence type (ST60) to be common to both Thailand and Australia, contradicting earlier studies showing complete distinction between isolates from these regions. The ST60 isolates reportedly from Australia had been obtained for MLST from UK and US collections. We have located and characterized the original Australian isolates; they were collected in 1983 and they are neither ST60 nor B. pseudomallei. The B. pseudomallei MLST database has been corrected and there is no ST common to isolates verified as from Australia or from Thailand.




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