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J. Clin. Microbiol. doi:10.1128/JCM.01720-06
Copyright (c) 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Structural shifts of mucosa-associated Lactobacilli and Clostridium leptum subgroup in patients with ulcerative colitis

Laboratory of Molecular Microbial Ecology and Ecogenomics, Shanghai Center for Systems Biomedicine, College of Life Science and Biotechnology, Shanghai Jiao tong University, 200240 Shanghai, China, and Central Hospital of Shanxi Coal Industry, 030006 Taiyuan, China

^* To whom correspondence should be addressed. Email: lpzhao{at}sjtu.edu.cn.

	Abstract

To understand the role of the mucosa-associated microbiota in pathogenicity of ulcerative colitis (UC), paired biopsies were obtained during colonoscopy from the ulcerated and non-ulcerated gut mucosa of 24 patients with UC. Denaturing gradient gel electrophoresis (DGGE) analysis was employed to profile the composition of the dominant bacteria (16S rRNA gene V3 region) and three important groups Lactobacilli, Clostridium leptum subgroup and Bacteroides spp.. Pearson coefficient was used to estimate the similarity of the bacterial community between the paired biopsies for each patient. The average similarity values of bacterial composition between the paired samples were 94.8±3.8% for dominant bacteria, 59.9±26.1% for Lactobacilli, 79.2±22.6% for Clostridium leptum subgroup and 88.7±16.4% for Bacteroides spp.. The data revealed that Lactobacilli and Clostridium leptum subgroup were significantly different between ulcerated and non-ulcerated regions. It also was noted that for Lactobacilli, the composition varied significantly between biopsy sites irrespective of the location of UC in the gut, but the composition of Clostridium leptum subgroup showed a significant difference between paired samples from UC in rectum and not in left colon. Localized dysbiosis of the mucosa-associated intestinal microflora, especially for Lactobacilli and Clostridium leptum subgroup may be closely related to UC.

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