JCM Accepts, published online ahead of print on 4 October 2006

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J. Clin. Microbiol. doi:10.1128/JCM.01887-06
Copyright (c) 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Rapid Screening of Topoisomerase Gene Mutations using a Novel Melting Curve Analysis for Early-Warning of Fluoroquinolone-Reistant Streptococcus pnuemoniae

Kazuko Y. Fukushima, Yoichi Hirakata^*, Kazuyuki Sugahara, Katsunori Yanagihara, Akira Kondo, Shigeru Kohno, and Shimeru Kamihira

Department of Laboratory Medicine, and Second Department of Internal Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki City 852-8501, Japan

^* To whom correspondence should be addressed. Email: hirakata{at}nagasaki-u.ac.jp.

We developed a real-time PCR assay combined with melting curve analysis for rapidly genotyping quinolone-resistant determining regions (QRDR) of topoisomerase genes in Streptococcus pneumoniae. This assay was not only accurate for the screening of fluoroquinolone (FQ) resistance but also relevant as an early-warning system of detecting pre-existent single QRDR mutations.

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