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J. Clin. Microbiol. doi:10.1128/JCM.01926-07
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

PROSPECTIVE STUDY OF HUMAN METAPNEUMOVIRUS DETECTION IN CLINICAL SAMPLES USING LIGHT DIAGNOSTICS^TM DIRECT IMMUNOFLUORESCENCE REAGENT AND REAL-TIME PCR

Department of Laboratory Medicine, Yale New Haven Hospital and Yale University School of Medicine, New Haven, CT

^* To whom correspondence should be addressed. Email: marie.landry{at}yale.edu.

	Abstract

A Light Diagnostics^TM human metapneumovirus (HMPV) monoclonal antibody reagent was evaluated using cytospin-enhanced direct immunofluorescence (DFA) and compared to TaqMan RT-PCR. Of 202 samples tested, 48 were positive by RT-PCR and 41 (85.4%) of these were positive by DFA (p=0.0771). Commercial availability of an HMPV DFA reagent will be of significant benefit to clinical laboratories.