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JCM Accepts, published online ahead of print on 30 January 2008
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JCM.02234-07v1
46/4/1534    most recent
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J. Clin. Microbiol. doi:10.1128/JCM.02234-07
Copyright (c) 2008, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved.

Identification of Staphylococcus aureus and Determination of Methicillin Resistance Directly from Positive Blood Cultures by Isothermal Amplification and Disposable Detection Device

James Goldmeyer, Haijing Li, Melinda McCormac, Steve Cook, Charles Stratton, Bertrand Lemieux, Huimin Kong, Wen Tang, and Yi-Wei Tang*

BioHelix Corporation, Beverly, MA 01915; Departments of Medicine and Pathology, Vanderbilt University Medical Center, Nashville, TN 37232; and Lahey Clinic Medical Center, Burlington, MA 01805

* To whom correspondence should be addressed. Email: yiwei.tang{at}vanderbilt.edu.


  Abstract

A simple, rapid, and user-friendly procedure has been developed to identify Staphylococcus aureus, and determine its methicillin resistance directly from Gram-positive cocci in clusters-containing blood culture medium. The specimens were diluted and heated prior to amplification of the nuc and mecA genes with isothermal helicase-dependent amplification. Amplicons were detected using a disposable detection device. The analytical sensitivity of the assays was 50 colony-forming units per reaction, and the clinical sensitivity and specificity were both 100% for S. aureus detection, and 100% and 98% for methicillin resistance determination, respectively.




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