Band fluorescence intensity as a function of carbohydrate concentration and oligomannoside length. (A) The relationship between band intensity and carbohydrate concentration was determined. Band fluorescence intensities of serial dilutions of maltose and maltotetraose were calculated and related to carbohydrate concentration for triplicate samples (r2 = 0.96140). (B) Nonpreferential ANTS labeling of maltooligosaccharides of various lengths was demonstrated. Aliquots of the maltooligosaccharide were derivatized under identical conditions for 0.5 to 12 h. The oligosaccharide concentration of the maltotetraose (G4) through maltonanose (G9) demonstrated an ANTS-labeling rate independent of oligomer length.
