Relative Abundance of Oligosaccharides in CandidaSpecies as Determined by Fluorophore-Assisted Carbohydrate Electrophoresis

Table 2.

RMIa of oligosaccharides released by whole-cell β-elimination of 16 Candida strains

RMI Band fluorescence intensity scoreb for:
C. albicans A9 C. albicans3153A C. glabrata2001 C. glabrataM33568 C. krusei 01 C. krusei 6258 C. lusitaniaeY1.190 C. lusitaniae64125 C. parapsilosisY1.797 C. parapsilosis22016 C. rugosa8.47 C. rugosa 8.48 C. stellatoidea 11006 C. stellatoidea36232 C. tropicalisY1.787 C. tropicalis Y1.802
1.00 1 1 2 3 2 2 2 2 2 2 3 3 2 2 1 1
1.18 3 3 4 4 4 4 4 4 4 4 4 4 4 4 4 4
1.55 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4
1.82 2 1 3 2 2
1.92 3 2 3 3 4 4 2 2 2 2 3 3 3 3 2 2
1.98 3 2 3 3 4 4 2 2 2 2 3 3 3 3 2 2
2.20 4 3 4 4 3 3 3 3 4 4 4 4 4 3 2 2
2.36 1 1
2.44 2 2 1 1 1 1
2.85 1 1 2 2
3.05 2 1 1 1 2 1
3.15 3 3 2 2
3.25 4 4 3 2
3.40 2 2
3.54 1 1 1 1 1
3.66 2 2
3.80 1 1 1
4.05 1 2 2 1 1
4.20 1 2 2 3 2 2
4.48 1
4.60 1 1 1 1 1 1 1 1
5.14 1 1 4 4 1 1
5.95 2 2 1 1
6.40 1 1
6.65 1 1 1 1
  • a Migration distance relative to that of maltooligosaccharides derived from dextran; e.g., a band 0.5 of the unit distance between glucose (RMI 1.00) and maltose (RMI 2.00) has an RMI of 1.50.

  • b Band fluorescence intensity was measured using SigmaGel analytical software as described in the text and rated as a percentage of pixel number ranging from background (0%) through saturation (100%): 1 = 0 to 25%; 2 = 26 to 50%; 3 = 51 to 75%; 4 = 76 to 100% saturation.

This Article

  1. J. Clin. Microbiol. vol. 38 no. 8 2862-2869